Background:

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

Applications:

WB

Name of antibody:

Rubisco

Immunogen:

Fusion protein of Arabidopsis thaliana Rubisco

Full name:

Ribulose-1,5-bisphosphate carboxylase/oxygenase

Synonyms:

Rubisco; RuBisCO large subunit; Ribulose bisphosphate carboxylase large chain

SwissProt:

O03042

WB Predicted band size:

53 kDa

WB Positive control:

Arabidopsis protein lysate

WB Recommended dilution:

5000-10000