FC Recommend dilution:

20-100

Background:

The abundance of glyceraldehyde-3-phosphate dehydrogenase in eukaryotic cells is relatively unaffected by eternal factors. In western blot analysis the level of GAPDH, a 38kDa protein, can be used as a reference value for loading control. Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Applications:

FC

Name of antibody:

FITC-conjugated GAPDH

Immunogen:

Fusion protein of human GAPDH

Full name:

Glyceraldehyde-3-phosphate dehydrogenase

Synonyms：

GAPD, G3PD, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH

SwissProt:

P04406

FC Recommended dilution:

20-100