Background:

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Applications:

ELISA, WB, IHC

Name of antibody:

MCAM

Immunogen:

Synthetic peptide of human MCAM

Full name:

melanoma cell adhesion molecule

Synonyms:

CD146; MUC18; HEMCAM; METCAM; MelCAM

SwissProt:

P43121

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human thyroid cancer and Human ovarian cancer

IHC Recommend dilution:

40-200

WB Predicted band size:

72 kDa

WB Positive control:

Human leiomyosarcoma tissue lysate

WB Recommended dilution:

500-2000